For Lab 3, you will complete two exercises:
Please review the Biology Laboratory Safety guidelines before you begin each laboratory investigation. Look at SCB115-Syllabus.pdf for accessibility and other policies.
Exercise 5 Content
Learning Objectives
After completing this lab, the students will be able to:
- Describe the four major types of biomolecules and their functions.
- Describe the basic subunits that comprise these molecules.
- Describe the tests used to identify these molecules.
- Distinguish between positive and negative control tests.
Lab Readings
Read the following materials before you begin the experiment.
- Chapter 3: Biological Macromolecules (source: Openstax)
- Module 3: Biological Macromolecules (Source: Lumen learning)
- Study Guide: Chemistry of Life Study Questions (Lumen Learning)
- Biological Molecules (Lumen learning)
- Ch 03_ Biological Macromolecules (OneDrive)
Videos
Watch the following videos on the scientific method before you start the lab activity.
- Watch below or on YouTube the video The molecules of Life
https://youtu.be/QWf2jcznLsY
- Watch below or on YouTube the video Biological Molecules
https://www.youtube.com/watch?v=PYH63o10iTE
Lab Activities
Watch these two lab simulation videos to detect the presence of each type of biological molecule.
- Lab Simulation – Biological Macromolecules video
https://wilmu.mediaspace.kaltura.com/media/Sci+251+-+Lab+Simulation+-+Biological+Macromolecules/0_ibkdoqzy
- Watch the video below or on YouTube Makin’ up labs with Stu Dent Ep 1 Biomolecules
- Test Results
- Benedict’s Test for reducing sugar
- https://microbiologyinfo.com/benedicts-test-principle-composition-preparation-procedure-and-result-interpretation/
- Iodine test for starch (slide no.5)
- Sudan III Test for lipid (please click free full text to view the lipid test result)
- https://europepmc.org/article/pmc/pmc3577220
- Biuret Test for Protein
- https://microbenotes.com/biuret-test-for-protein/
- Benedict’s Test for reducing sugar
Quiz
Test (link to OneDrive)
This test is from quizlet.com, and can be obtained there.