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Exercise 9 Content

• Learning Objectives
• Lab Readings
• Videos
• Lab Activity
• Quiz

Learning Objectives

After completing this lab, the students will be able to:

• define diffusion and osmosis.
• explain the relationship between rate of diffusion and molecular weight
• Predict the movement of water (or other substances) across semi-pemeable membranes.
• Recognize hypertonic, hypotonic and isotonic solutions.
• Predict changes in living cells placed in a hypertonic, hypotonic, or isotonic solution.

Lab Readings

Read the following materials before you begin the experiment.

1. 5.2: Passive Transport contributed by Boundless (Libretext).
   5.2A: The Role of Passive Transport
   5.2B: Selective Permeability
   5.2C: Diffusion
   5.2D: Facilitated transport
   5.2E: Osmosis
   5.2F: Tonicity
   
2. Passive Transport: Filtration and Facilitated Diffusion by Barbara Liang.
   Click Next to start the slide.
   https://www.wisc-online.com/learn/general-education/anatomy-and-physiology1/ap11103/passive-transport-filtration-and-facilitated
   Passive transport: osmosis by Barbara Liang.
   https://www.wisc-online.com/learn/natural-science/life-science/ap11003/the-cell-passive-transport-osmosis
   
3. Chapter 7-3 The Cell Membrane; Active and Passive transport (21 slides).
   
4. https://microbenotes.com/osmosis-and-diffusion/ by Anupama Sapkota.
5. Passive transport, active transport, Bulk transport (Openstax).
6. Active transport: Endocytosis, Exocytosis, and Sodium-potassium pump.
   https://www.biologycorner.com/bio1/notes_active_transport.html
   
   

Lab Activity

in this lab activities, you will observe how to conduct these experiments in the lab.

1. Diffusion Methylene blue through Agar Gel.
   https://www.biophysics.org/Portals/0/BPSAssets/Education/Documents/LessonPlanDiffusion_122115sm.pdf 
2. Diffusion Potassium Permanganate, Methylene Blue, and Safranin after 30 minute.
   https://www.youtube.com/watch?v=RdJsu4S56mM&feature=youtu.be

3. Cell membrane model demonstration using dialysis tubing on you tube.
https://www.youtube.com/watch?v=2CNGe3jMej0
4. Dialysis Tubing Diffusion Time-lapse on you tube.
https://www.youtube.com/watch?v=kp9EvMeTMUs&feature=emb_logo
5. Osmosis Demo. Time lapse start at 3.50. (Bozeman Science)
https://www.youtube.com/watch?v=eQsAzXr0UCU&feature=youtu.be
6. Onion Osmosis happened after salt solution is added.
https://www.youtube.com/watch?v=S29InN1YSl4&feature=youtu.be

7. Red blood cells under the microscope, hypo and hypertonic solutions.
https://www.youtube.com/watch?v=A8cI6FkcG4c&feature=emb_logo

Quiz

1. Diffusion Quiz (6 questions)
   https://www.biologycorner.com/quiz/qz_diffusion.html
2. Cell transport quiz.
   https://www.unmisravle.com/passive-and-active-transport-worksheets/passive-and-active-transport-worksheet-worksheets-for-all-2/